SPR分子相互作用仪



一、型号/Model:SPR-T200, SPR-T210, SPR-I300

二、技术参数/Specifications:

型号/Model SPR-T200 SPR-T210 SPR-I300
光源
Light Source
类型/Type He-Ne Laser He-Ne Laser He-Ne Laser,and LED
波长/wavelength 632.8nm 632.8nm 632.8nm,and 621±10nm
转台
Rotation Stage
转台数量
Number of Rotation stage
Motor 1, sample motor
Motor 2, detector motor
Motor 1, sample motor
Motor 2, detector motor
Motor 1, sample motor
Motor 2, detector motor
转台控制
Control of rotation stage
软件自动控制
Automatically control
软件自动控制
Automatically control
软件自动控制
Automatically control
转台角度精度
Rotation angular resolution
<= 0.02°
可升级/upgradable
0.01°
可升级/upgradable
0.01°
可升级/upgradable
转台旋转角度范围
Rotation angles
0-360°(Motor 1)
0-180°(Motor 2)
0-360°(Motor 1)
0-180°(Motor 2)
0-360°(Motor 1)
0-180°(Motor 2)
最佳测量角度范围
Optimal measurable angles
15-80°(incident angle)
(for prism 1.845)
15-80°(incident angle)
(for prism 1.845)
15-80°(incident angle)
(for prism 1.845)
转台防撞系统
Anti-collision system
两个行程开关 AZ-721, detector motor 超出 0-180°范围时关闭电机2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. 两个行程开关 AZ-721, detector motor 超出 0-180°范围时关闭电机2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. 两个行程开关 AZ-721, detector motor 超出 0-180°范围时关闭电机2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°.
角度校准
Angular calibration
半自动
Semi-automatic
半自动
Semi-automatic
半自动
Semi-automatic
载物台
Stage
平台控制类型
Motion Type
手动
Manual
手动
Manual
手动
Manual
移动平台
Translation stage
XYZ 移动平台 XYZ 移动平台 XYZ 移动平台
检测器
Detector
光检测器
Photo detector
光电二极管
Photo-diode detector
光电二极管
Photo-diode detector
光电二极管+CCD相机
A photo-diode detector, and a CCD camera
流通系统
Flow Cell System
进样系统
Injection system
蠕动泵
Peristaltic Pump
蠕动泵
Peristaltic Pump
蠕动泵
Peristaltic Pump
型号 Type BT100-2J BT100-2J BT100-2J
通道数
Pump channels
2 2 2 or 4(Optional)
进样控制
Injection Control
自动和手动均可
Both Automatic and Manual
自动和手动均可
Both Automatic and Manual
自动和手动均可
Both Automatic and Manual
进样体积
Injection Volume
20-750 μl 20-750 μl 20-750 μl
流通池容量
Flow Cell Capacity
20-100 μl 20-100 μl 20-100 μl
流通通道数量
Flow Cell Number
单通道
Single Channel
单通道
Single Channel
1-4通道(可选)
1-4 Channels (Optional)
基本参数
Basic Parameters
设备尺寸
Instrument Size
1120 mm*560 mm*560 mm 1120 mm*560 mm*500 mm 1100 mm*560 mm*500 mm
设备重量
Instrument Weight
约120 kg 约120 kg 约120 kg
折射率灵敏度
Sensitivity
10-5-10-6 RIU
Depends on the sensor chips
10-6 RIU
Depends on the sensor chips
10-6 RIU
Depends on the sensor chips
配件
Accessories
棱镜
Prism
折射率/Refractive index, 1.745或1.845
可调换/Exchangeable
折射率/Refractive index, 1.745或1.845
可调换/Exchangeable
折射率/Refractive index, 1.745或1.845
可调换/Exchangeable
流通池
Flow cell
Quartz and PDMS Quartz and PDMS Quartz and PDMS

Tubes
2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm
2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm
2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm
匹配油
Matching Oil
1.7000±0.0002
Cargille Labs
1.7000±0.0002
Cargille Labs
1.7000±0.0002
Cargille Labs
样品芯片
Sample Chips
Sensor chips with 50 nm Au
可定制/Customizable
Sensor chips with 50 nm Au
可定制/Customizable
Sensor chips with 50 nm Au
可定制/Customizable
其他
Others
电源
Power Supply
220V/50 HZ AC 220V/50 HZ AC 220V/50 HZ AC
软件
Software
Sensby SPR Sensby SPR Sensby SPR
操作系统
System
Windows 7, 10 Windows 7, 10 Windows 7, 10

三、设备可以实现功能/Functions of SPR
  仪器 SPR 是基于表面等离子体共振(SPR)技术来实时跟踪生物分子间的相互作用,不需任何标记物的技术。该设备通过固定波长,测量角度反射光谱获得分子相互作用信息;也可固定入射角度,测量分子相互作用动力学曲线。SPR可进行:1、动力学常数的测定;2、样品浓度的测定;3、分析相互作用模式;4、复合物功能分析。

The instrument SPR is based on Surface Plasmon Resonance (SPR) technology to monitor the interaction of biomolecules in real time. The molecular interactions information is obtained by measuring the angular reflection spectroscopy at a fixed wavelength; or measuring the kinetic of molecular interactions through reflectivity intensity changes at a fixed incident angle. SPR is able to perform the determination of kinetic constant, detection of sample concentration; analysis of the interaction modes and functional analysis of complex.


四、设备的主要应用领域/Main Application Field of SPR
蛋白质组学研究(Proteomics) 免疫测定法(Immunoassay)
新药研发(Drug Discovery) 疫苗开发(Vaccine Development)
癌症研究(Cancer Research) 瞬时结合(Transient Binding)
信号传递 (Cell Signaling ) 配体垂钓(Ligand Fishing)
多分子复合物的结构和组装( Multi-molecular Complexes) 结合特异性(Binding Specificity)
分子识别(Molecular Recognition) 结构与功能的关系(Structure-function Relationship)
免疫调节(Immune Regulation) 酶反应(Enzyme Reaction)

五、基本原理/Principle
  如图所示,SPR 分子相互作用仪器的原理是波长 632.8 nm 的激光形成的 p 偏振光,到达棱镜后激发金属表面的表面等离子体发生共振。通过改变入射光角度 θ,实时检测反射光强度的变化 R,根据反射光强度变化 ΔR 或共振角度的变化 Δθ,实现分子相互作用的检测;也可以通过固定角度,测量反射光强的变化曲线获得分子相互作用动力学常数。

As shown in the figure, the laser at the wavelength of 632.8 nm passes through a polarizer to become a transverse magnetically polarized beam and is coupled to glass prism to excite surface plasmon resonance of sensor chip. The reflected light intensity R is detected in real time by the changes of incident light angle θ. The detection of molecular interactions can be achieved by the changes of ΔR or Δθ; the molecular interaction kinetic constants can also be obtained by measuring the reflectivity changes at a fixed angle.

六、资料下载
森佰SPR-T200说明书
SPR T200 software

型号/Model SPR-T210 SPR-I400
检测器
Detector
光检测器
Photo detector
光电二极管
Photo-diode detector
CCD
CCD
流通系统
Flow Cell System
进样系统
Injection system
蠕动泵
Peristaltic Pump
注射泵
Peristaltic Pump
进样控制
Injection Control
半自动
Semi-Automatic
自动
Automatic
样品台
Sampling
-- 96孔板
自动切换
进样体积
Injection Volume
20-750 μl 20-750 μl
流通池容量
Flow Cell Capacity
20-100 μl 20-100 μl
流通通道数量
Flow Cell Number
单通道
Single Channel
4通道
4 Channels
基本参数
Basic Parameters
设备尺寸
Instrument Size
1120 mm*560 mm*500 mm 850 mm*630 mm*611 mm
设备重量
Instrument Weight
约120 kg 约50 kg
样品折射率测量范围
Measurable Sample Refractive Index
1~1.5 1~1.5
折射率灵敏度
Sensitivity
~10-6 RIU
Depends on the sensor chips
~10-6 RIU
Depends on the sensor chips
浓度定量灵敏度
Sensitivity for Concentration Detection
10 ng/ml 10 ng/ml
定量范围
Detection Range
0.1 μg/ml – 5 mg/ml 0.1 μg/ml – 5 mg/ml
检测浓度范围
Detection Range
0.05 nM-1 mM 0.05 nM-1 mM
检测解离常数(KD)范围
KD Range
0.05 nM-0.1 mM 0.05 nM-0.1 mM
配件
Accessories
棱镜
Prism
折射率/Refractive index, 1.845
可调换/Exchangeable
折射率/Refractive index, 1.845
可调换/Exchangeable
流通池
Flow cell
Quartz and PDMS Quartz and PDMS

Tubes
2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm
2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm
匹配油
Matching Oil
1.7000±0.0002
Cargille Labs
1.7000±0.0002
Cargille Labs
样品芯片
Sample Chips
Sensor chips with 50 nm Au
可定制/Customizable
Sensor chips with 50 nm Au
可定制/Customizable
其他
Others
电源
Power Supply
220V/50 HZ AC 220V/50 HZ AC
软件
Software
Sensby SPR Sensby SPR
操作系统
System
Windows 7, 10 Windows 7, 10